Avoidance of APOBEC3B-induced mutation by error-free lesion bypass
نویسندگان
چکیده
منابع مشابه
Avoidance of APOBEC3B-induced mutation by error-free lesion bypass
APOBEC cytidine deaminases mutate cancer genomes by converting cytidines into uridines within ssDNA during replication. Although uracil DNA glycosylases limit APOBEC-induced mutation, it is unknown if subsequent base excision repair (BER) steps function on replication-associated ssDNA. Hence, we measured APOBEC3B-induced CAN1 mutation frequencies in yeast deficient in BER endonucleases or DNA d...
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Error-free lesion bypass and error-prone lesion bypass are important cellular responses to DNA damage during replication, both of which require a DNA polymerase (Pol). To identify lesion bypass DNA polymerases, we have purified human Polκ encoded by the DINB1 gene and examined its response to damaged DNA templates. Here, we show that human Polκ is a novel lesion bypass polymerase in vitro. Puri...
متن کاملMolecular insights into error-prone DNA replication and error-free lesion bypass.
6·4 pp CPD error propensity when dealing with undamaged DNA, which distinguishes them from known high-fidelity DNA polymerases. Most of their biological roles and functions have not been elucidated yet. Nevertheless, recent investigations suggest the involvement of pol L together with poll;, in error-prone translesion DNA synthesisPJ Furthermore, it has been shown that pol L possesses lyase act...
متن کاملError-prone lesion bypass by human DNA polymerase eta.
DNA lesion bypass is an important cellular response to genomic damage during replication. Human DNA polymerase eta (Pol(eta)), encoded by the Xeroderma pigmentosum variant (XPV) gene, is known for its activity of error-free translesion synthesis opposite a TT cis-syn cyclobutane dimer. Using purified human Pol(eta), we have examined bypass activities of this polymerase opposite several other DN...
متن کاملStepwise Translocation of Dpo4 Polymerase during Error-Free Bypass of an oxoG Lesion
7,8-dihydro-8-oxoguanine (oxoG), the predominant lesion formed following oxidative damage of DNA by reactive oxygen species, is processed differently by replicative and bypass polymerases. Our kinetic primer extension studies demonstrate that the bypass polymerase Dpo4 preferentially inserts C opposite oxoG, and also preferentially extends from the oxoG*C base pair, thus achieving error-free by...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2017
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkx169